pH Corrections and Protein Ionization in Water/Guanidinium Chloride

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pH corrections and protein ionization in water/guanidinium chloride.

More than 30 years ago, Nozaki and Tanford reported that the pK values for several amino acids and simple substances in 6 M guanidinium chloride differed little from the corresponding values in low salt (Nozaki, Y., and C. Tanford. 1967. J. Am. Chem. Soc. 89:736-742). This puzzling and counter-intuitive result hinders attempts to understand and predict the proton uptake/release behavior of prot...

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ژورنال

عنوان ژورنال: Biophysical Journal

سال: 2001

ISSN: 0006-3495

DOI: 10.1016/s0006-3495(01)75980-2